Human EGFR / HER1 / ErbB1 Protein (His Tag)
ERBB,ERBB1,HER1,mENA,NISBD2,PIG61
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| Catalog Number | P10001-H08B |
|---|---|
| Organism Species | Human |
| Host | Baculovirus-Insect Cells |
| Synonyms | ERBB,ERBB1,HER1,mENA,NISBD2,PIG61 |
| Molecular Weight | The recombinant human EGFR consists of 626 amino acids and has a calculated molecular mass of 69.2 kDa. The recombinant protein migrates as an approximately 75 kDa protein in SDS-PAGE under reducing conditions. |
| predicted N | Leu 25 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain (Met 1-Lys 642) of human EGFR (NP_005219) was expressed with a C-terminal polyhistidine tag. |
| Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized human EGF at 10 μg/ml can bind human EGFR with a linear range of 3.2-400 ng/ml . |
| Research Area | Signaling |Signal Transduction |Protein Phosphorylation |Tyrosine Kinase |Receptor Tyrosine Kinases |
| Formulation | Lyophilized from sterile 20mM Tris, 50mM NaCl, 3mM DTT, 0.5mM TCEP, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently. |
| Reference |