Human ENO1 / Enolase 1 / alpha-enolase Protein (His Tag)

ENO1L1,HEL-S-17,MPB1,NNE,PPH

Catalog Number	P11554-H07E
Organism Species	Human
Host	E. coli
Synonyms	ENO1L1,HEL-S-17,MPB1,NNE,PPH
Molecular Weight	The recombinant human ENO1 consisting of 450 amino acids and has a calculated molecular mass of 49.3 kDa. It migrates as an 47 kDa band in SDS-PAGE under reducing conditions as predicted.
predicted N	Met
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human ENO1 (P06733-1) (Met 1-Lys 434) was expressed, with a polyhistide tag at the N-terminus.
Bio-activity
Research Area	Epigenetics \|Transcription \|Cancer susceptibility \|Tumor Suppressor
Formulation	Lyophilized from sterile 20mM Tris, 10% glycerol, pH 8.5 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Capello M, et al. (2011) a-Enolase: a promising therapeutic and diagnostic tumor target. FEBS J. 278(7): 1064-74. Kang HJ, et al. (2008) Structure of human alpha-enolase (hENO1), a multifunctional glycolytic enzyme. Acta Crystallogr D Biol Crystallogr. 64(Pt 6): 651-7. Lopez-Alemany R, et al. (2005) Alpha-enolase plasminogen receptor in myogenesis. Front Biosci. 10: 30-6. Ejeskdr K, et al. (2005) Introduction of in vitro transcribed ENO1 mRNA into neuroblastoma cells induces cell death. BMC Cancer. 5: 161.