Human ENO3 / beta-enolase Protein (His Tag)

ENO3

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Catalog Number	P14270-H07E
Organism Species	Human
Host	E. coli
Synonyms	ENO3
Molecular Weight	The recombinant human ENO3 consists of 449 amino acids and predicts a molecular mass of 48.8 KDa. It migrates as an approximately 45 KDa band in SDS-PAGE under reducing conditions.
predicted N	His
SDS-PAGE
Purity	> 85 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mature form of human ENO3 (AAH17249.1) (Met1-Lys434) was expressed with a polyhistide tag at the N-terminus.
Bio-activity
Research Area	Developmental Biology |Metabolism |Pathways and Processes |Metabolic signaling pathways |Carbohydrate metabolism
Formulation	Lyophilized from sterile 50mM Tris, 0.1% Brij35, pH 8.0. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	ENO3 is one of the three enolase isoenzymes found in mammals. As a homodimer, ENO3 is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in ENO3 gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for ENO3 gene that differ only in their 5' UTR. ENO3 may play a role in muscle development and regeneration. It appears to have a function in striated muscle development and regeneration.
Reference	Peshavaria M, et al. (1989) Structure of human muscle (beta) enolase mRNA and protein deduced from a genomic clone. Nucleic Acids Res. 17(21):8862. Calì L, et al. (1990) Nucleotide sequence of a cDNA encoding the human muscle-specific enolase (MSE). Nucleic Acids Res. 18(7):1893. Peshavaria M, et al. (1991) Molecular structure of the human muscle-specific enolase gene (ENO3). Biochem J. 275(2):427-33.