Human ENTPD5 Protein (His Tag)
CD39L4,NTPDase-5,PCPH
- 100ug (NPP3827) Please inquiry
| Catalog Number | P11184-H08B |
|---|---|
| Organism Species | Human |
| Host | Baculovirus-Insect Cells |
| Synonyms | CD39L4,NTPDase-5,PCPH |
| Molecular Weight | The secreted recombinant human ENTPD5 consists of 418 amino acids and predicts a molecular mass of 47 kDa. It migrates as an approximately 45 KDa band in SDS-PAGE in SDS-PAGE under reducing conditions. |
| predicted N | Val 21 |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human ENTPD5 (O75356) (Met 1-His 428) was fused with a polyhistidine tag at the C-terminus. |
| Bio-activity | Measured by its ability to hydrolyze the 5’phosphate groups from the substrate guanosine5’diphosphate (GDP). The specific activity is >7,000 pmoles/min/μg. |
| Research Area | Cancer |Signal transduction |Metabolism |Types of disease |Metabolism in Cancer |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Ectonucleoside triphosphate diphosphohydrolase 5 (ENTPD5), also known as CD39 antigen-like 4, ER-UDPase, Guanosine-diphosphatase ENTPD5, Nucleoside diphosphatase Uridine-diphosphatase ENTPD5. This hydrolase is expressed in response to phosphoinositide 3-kinase (PI3K) signaling. Activation of PI3K results in FOXO phosphorylation by AKT1 and loss of ENTPD5 transcriptional repression. It is Up-regulated in PTEN-deficient cells. Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation.ENTPD5 promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, ENTPD5 constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. ENTPD5 also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. This enzyme Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling. |
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