Human ERN1 / IRE1 Protein (aa 465-977, His & GST Tag)

hIRE1p,IRE1,IRE1a,IRE1P

Catalog Number	P11905-H20B
Organism Species	Human
Host	Baculovirus-Insect Cells
Synonyms	hIRE1p,IRE1,IRE1a,IRE1P
Molecular Weight	The recombinant human ERN1/GST chimera consists of 750 amino acids and has a calculated molecular mass of 86 kDa. It migrates as an approximately 90 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 80 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human ERN1 isoform 1 (O75460-1) cytoplasmic domain (Pro 465-Leu 977) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Bio-activity	1. Kinase activity untested 2. Measured by its nuclease activity to cleave Xbp1 single stem-loop mini-substrate.
Research Area	Microbiology \|Pathogenic microorganism \|viruses \|animal virus \|viral illness \|Viral tract respiratory illness \|
Formulation	Supplied as sterile 20mM Tris, 500mM NaCl, 10% gly, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Trentmann,S.M. et al., 2000, Plant Mol Biol. 44 (1):11-25. Iwawaki T., et al., 2001, Nat. Cell Biol. 3:158-64. Liu C.Y., et al., 2003, J. Biol. Chem. 278:17680-7. Oppermann F.S., et al., 2009, Mol. Cell. Proteomics 8:1751-64.