Human EZR / VIL2 / Ezrin Protein (His Tag)

CVIL,CVL,HEL-S-105,VIL2

• 100ug (NPP2123) Please inquiry

Catalog Number	P12858-H07E
Organism Species	Human
Host	E. coli
Synonyms	CVIL,CVL,HEL-S-105,VIL2
Molecular Weight	The recombinant human EZR consists of 601 amino acids and predicts a molecular mass of 71.2 KDa. It migrates as an approximately 82 KDa band in SDS-PAGE under reducing conditions.
predicted N	His
SDS-PAGE
Purity	> 60 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mature form of human EZR (P15311) (Pro2-Leu586) was expressed with a polyhistide tag at the N-terminus.
Bio-activity
Research Area	Immunology |Signal Transduction |Cytoskeleton / ECM |Cytoskeletal Proteins |Microfilaments |Actin etc |
Formulation	Lyophilized from sterile PBS 100mM Arg 10% glycerol, 0.1% Twen 20, pH 7.5 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	VIL2, also known as villin-2, belongs to the ERM protein family. It is expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. VIL2 contains 1 FERM domain and may be involved in connections of major cytoskeletal structures to the plasma membrane. It plays a key role in cell surface structure adhesion, migration, and organization. In epithelial cells, VIL2 is required for the formation of microvilli and membrane ruffles on the apical pole. It interacts with MPP5 and SLC9A3R2. It is found VIL2 form a complex with PODXL and SLC9A3R2. It also interacts with MCC, PLEKHG6, PODXL, SCYL3/PACE1, SLC9A3R1 and TMEM8B.
Reference	Grnholm M, et al. (1999) Homotypic and heterotypic interaction of the neurofibromatosis 2 tumor suppressor protein merlin and the ERM protein ezrin. J Cell Sci. 112(6):895-904. Sitaraman, et al. (2002) The adenosine 2b receptor is recruited to the plasma membrane and associates with E3KARP and Ezrin upon agonist stimulation. J Biol Chem. 277(36):33188-95. Gould KL, et al. (1989) cDNA cloning and sequencing of the protein-tyrosine kinase substrate, ezrin, reveals homology to band 4.1. EMBO J. 8(13):4133-42.