Human EphB4 / HTK Protein (His Tag)
HTK,MYK1,TYRO11
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| Catalog Number | P10235-H08H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | HTK,MYK1,TYRO11 |
| Molecular Weight | The recombinant human EphB4 is a monomeric protein. It consists of 535 amino acids and has a calculated molecular mass of 58.5 kDa. As a result of glycosylation, the rhEphB4 migrates as an approximately 72 kDa protein in SDS-PAGE under reducing conditions. |
| predicted N | Leu 16 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain (Met 1-Ala 539) of human EphB4 (NP_004435.3) precursor was expressed with a C-terminal polyhistidine tag. |
| Bio-activity | Measured by its binding ability in a functional ELISA . Immobilized human EphB4 at 2 μg/ml (100 μl/well) can bind human EphrinB2 with a linear range of 1-25 ng/ml. |
| Research Area | Epigenetics |Transcription |Cancer susceptibility |Tumor Suppressor |
| Formulation | Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers. |
| Reference |