Human FGF18 / FGF-18 Protein (His Tag)

FGF-18,ZFGF5

100ug (NPP1278) Please inquiry

Catalog Number	P13206-H08H
Organism Species	Human
Host	Human Cells
Synonyms	FGF-18,ZFGF5
Molecular Weight	The secreted recombinant human FGF18 comprises 191 amino acids and has a predicted molecular mass of 22.3 kDa. The apparent molecular mass of rh FGF18 is approximately 32 kDa in SDS-PAGE under reducing conditions due to glycosylation.
predicted N	Glu 28
SDS-PAGE
Purity	> 92 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human FGF18 (O76093) (Met 1-Ala 207) was fused with a polyhistidine tag at the C-terminus.
Bio-activity	1. Measured by its binding ability in a functional ELISA. 2. Immobilized human FGF18 (P 13206-H08H) at 10 μg/mL (100 μl/well) can bind Rat FGFR4 (P80093-R02H), The EC₅₀ of Rat FGFR4 (P80093-R02H) is 1.17 μg/mL.
Research Area	Cancer \|Oncoprotein & suppressor & biomarker \|Oncoprotein \|Growth Factor & Receptor \|Fibroblast Growth Factor (FGF) & Receptor \|Fibroblast Growth Factor (FGF) \|
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Fibroblast growth factor 18 (FGF18) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. It has been shown in vitro that FGF18 is able to induce neurite outgrowth in PC12 cells. Studies of the similar proteins in mouse and chick suggested that this protein is a pleiotropic growth factor that stimulates proliferation in a number of tissues, most notably the liver and small intestine. Experiment datas identified FGF18 as a selective ligand for FGFR3 in limb bud mesenchymal cells, which suppressed proliferation and promoted their differentiation and production of cartilage matrix. FGF18 appears to regulate cell proliferation and differentiation positively in osteogenesis and negatively in chondrogenesis.
Reference	Ohbayashi N, et al. (2002) FGF18 is required for normal cell proliferation and differentiation during osteogenesis and chondrogenesis. Genes Dev. 16(7): 870-9. Davidson D, et al. (2005) Fibroblast growth factor (FGF) 18 signals through FGF receptor 3 to promote chondrogenesis. J Biol Chem. 280(21): 20509-15. Liu Z, et al. (2007) FGF18 is required for early chondrocyte proliferation, hypertrophy and vascular invasion of the growth plate. Dev Biol. 302(1): 80-91.