Human Fetuin-A / AHSG / FETUA Protein (His Tag)
A2HS,AHS,FETUA,HSGA
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| Catalog Number | P10318-H08H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | A2HS,AHS,FETUA,HSGA |
| Molecular Weight | The secreted recombinant human Fetuin A consists of 360 amino acids and predicts a molecular mass of 38.8 kDa. By SDS-PAGE under reducing conditions, rhFetuinA migrates as an approximately 55-60 kDa protein due to glycosylation. |
| predicted N | Ala 19 |
| SDS-PAGE |  |
| Purity | > 97 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human Fetuin A (NP_001613.2) (Met 1-Val 367) precursor was expressed with a C-terminal polyhistidine tag. |
| Bio-activity | 1. Measured by its binding ability in a functional ELISA. Immobilized human FCN1 at 10 μg/ml can bind biotinylated recombinant human Fetuin-A with a linear range of 16-2000 ng/ml. 2. Measured by its ability to inhibit active Cathepsin V cleavage of a fluorogenic peptide substrate Z-LR-AMC, R&D Systems, Catalog # ES008. The IC50 value is < 100 nM. |
| Research Area | Cardiovascular |Blood |Serum Protein |
| Formulation | Lyophilized from sterile PBS, pH 7.2 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Fetuin-A, also known as Alpha-2-HS-Glycoprotein (AHSG), belongs to the Fetuin family, is a plasma binding protein, and is more abundant in fetal than adult blood. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. Fetuins are carrier proteins like albumin. Fetuin-A forms soluble complexes with calcium and phosphate and thus is a carrier of otherwise insoluble calcium phosphate. Thus Fetuin-A is a potent inhibitor of pathological calcification. The circulating levels of fetuin-A, a well-described inhibitor of calcification, regulate the cell-dependent process of osteogenesis. The low circulating fetuin-A levels are associated with a greater prevalence and/or severity of Vascular calcification (VC) and increased risk for all-cause and cardiovascular mortality. However, high circulating fetuin-A levels appear to induce insulin resistance and, in non-dialyzed subjects with diabetic nephropathy, are directly related to VC burden. The emerging role of fetuin-A deficiency as a risk factor in dialysis patients was documented in cross-sectional studies demonstrating a significant correlation with all-cause and cardiovascular mortality. Additionally, Human fetuin-A is a negative acute phase protein involved in inflammatory diseases, thus being a potential physiological regulator of meprin activity. Fetuin-A is a broad-range protease inhibitor. Fetuin-A and cystatin C as endogenous proteolytic regulators of meprin activity broadens our understanding of the proteolytic network in plasma. |
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