Human Flavokinase / RFK Protein (His Tag)
RIFK
- 100ug (NPP2464) Please inquiry
| Catalog Number | P13881-H07E |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | RIFK |
| Molecular Weight | The recombinant human RFK consists of 170 amino acids and predicts a molecular mass of 19.5 KDa. It migrates as an approximately 20 KDa band in SDS-PAGE under reducing conditions. |
| predicted N | His |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mature form of human RFK (Q969G6) (Met1-His155) was expressed with a polyhistide tag at the N-terminus. |
| Bio-activity | Kinase activity untested |
| Research Area | Signaling |Signal Transduction |Metabolism |Types of disease |Metabolism in Cancer |
| Formulation | Supplied as sterile 20mM Tris, 10% glycerol, pH 8.0 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Flavokinase is a member of the transferases family, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. Flavokinase is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis. It has been proposed that TNF, through the activation of the flavokinase gene, enhances the incorporation of FAD in NADPH oxidase enzymes, which is a critical step for the assembly and activation of NADPH oxidase. |
| Reference |