Human GAPDH Protein (His Tag)
G3PD,GAPD,HEL-S-162eP
- 100ug (NPP2151) Please inquiry
| Catalog Number | P10094-H07E |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | G3PD,GAPD,HEL-S-162eP |
| Molecular Weight | The recombinant human GAPDH comprises 346 amino acids and migrates as an approximately 38 kDa band in SDS-PAGE under reducing conditions as predicted. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 93 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human GAPDH (P04406) (Met 1-Glu 335) was expressed, with a polyhistidine tag at the N-terminus. |
| Bio-activity | |
| Research Area | Immunology |Signal Transduction |Akt Pathway |Adaptor Proteins in the Akt Pathway |
| Formulation | Lyophilized from sterile 50mM Tris, 30% glycerol, pH 7.5 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions. |
| Reference |