Human GFPT1 / GFAT Protein

CMSTA1,GFA,GFAT,GFAT1,GFAT1m,GFPT,GFPT1L,MSLG

• 100ug (NPP3888) Please inquiry

Catalog Number	P11071-HNAE
Organism Species	Human
Host	E. coli
Synonyms	CMSTA1,GFA,GFAT,GFAT1,GFAT1m,GFPT,GFPT1L,MSLG
Molecular Weight	The recombinant human GFAT exists as a homotetramer protein. The GFAT monomer consists of 370 amino acids and predicts a molecular mass of 41.5 kDa as estimated in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 97 % as determined by SDS-PAGE
Protein Construction	The sequence corresponding to amino acids (Gln 332-Glu 699) of human GFAT (AAA58502.1) was expressed and purified with two amino acids (Gly & Pro) at the N-terminus.
Bio-activity
Research Area	Neuroscience |Neurotransmitter |Amino Acids
Formulation	Lyophilized from sterile PBS, pH 7.4, 10% glycerol 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Glutamine:fructose-6-phosphate amidotransferase 1 (GFAT), also known as GFPT1, is a member of the N-terminal nucleophile aminotransferases and the first rate-limiting enzyme for the entry of glucose into the hexosamine biosynthesis pathway (HBP) in mammals. GFAT transfers the amino group from the L-glutamine amide to the D-fructose 6-phosphate, producing glutamic acid and glucosamine 6-phosphate. GFAT exists as a homotetramer in cytoplasm, and is proposed to be most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. The full length of human GFAT contains 1 glutamine amidotransferase type-2 domain which catalyzes amide nitrogen transfer from glutamine to the appropriate substrate, and 2 SIS (Sugar Isomerase) domains found in many phosphosugar isomerases and phosphosugar binding proteins.Two isoforms of gfat have been identified: GFAT1 is predominantly expressed in skeletal muscle, whereas GFAT2 is expressed mainly in the central nervous system.
Reference