Human GPR37 Protein (His Tag)

EDNRBL,hET(B)R-LP,PAELR

• 100ug (NPP2169) Please inquiry

Catalog Number	P13496-H08H
Organism Species	Human
Host	Human Cells
Synonyms	EDNRBL,hET(B)R-LP,PAELR
Molecular Weight	The secreted recombinant human GPR37 comprises 250 amino acids and has a predicted molecular mass of 26.6 kDa. ? The apparent molecular mass of rh GPR37 is approximately 30-45 kDa in SDS-PAGE under reducing conditions due to different glycosylation.
predicted N	Ala 27
SDS-PAGE
Purity	> 80 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human GPR37 (NP_005293.1) the first extracellular domaqin (Met 1-Met 265) was fused with a polyhistidine tag at the C-terminus.
Bio-activity
Research Area	Immunology |Signal Transduction |Signaling Pathway |G Protein Signaling |G Protein-Coupled Receptors (GPCRs) |Orphan G Protein-Coupled Receptors |
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	GPR37 (cathepsin Z) is an orphan receptor which belongs to the G-protein coupled receptor 1 family. G protein coupled receptors is a large protein family comprised by transmembrane receptors that sense molecules outside the cell and activate inside signal transduction pathways and, ultimately, cellular responses. They only exists in eukaryotes, including yeast, choanoflagellates, and animals. These receptors are binded and activated by light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters. These ligands vary in size from small molecules to peptides to large proteins. G protein-coupled receptors are involved in many diseases, and are also the target of approximately 40% of all modern medicinal drugs. GPR37 is expressed in brain and spinal cord, and at lower levels in testis, placenta and liver, but no detectable expression observed in any other tissue. GPR37 may have a unique functional role in the central nervous system.
Reference	Marazziti D, et al. (1997) Cloning of GPR37, a gene located on chromosome 7 encoding a putative G-protein-coupled peptide receptor, from a human frontal brain EST library. Genomics. 45(1):68-77. Imai, et al. (2002) CHIP is associated with Parkin, a gene responsible for familial Parkinson's disease, and enhances its ubiquitin ligase activity. Mol Cell. 10(1):55-67. Imai, et al. (2001) An unfolded putative transmembrane polypeptide, which can lead to endoplasmic reticulum stress, is a substrate of Parkin. Cell. 105(7):891-902.