Human Galactolipase / PNLIPRP2 Protein (His Tag)

PLRP2

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Catalog Number	P13687-H08B
Organism Species	Human
Host	Human Cells
Synonyms	PLRP2
Molecular Weight	The secreted recombinant human PNLIPRP2 consists of 462 amino acids and predicts a molecular mass of 51.5 KDa. The apparent molecular mass of the protein is approximately 57 Kda in SDS-PAGE under reducing conditions due to glycosylation.
predicted N	Lys 18
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human PNLIPRP2 (P54317) (Met 1-Cys469) was expressed, with a C-terminal polyhistidine tag.
Bio-activity
Research Area	Immunology |Signal Transduction |Metabolism |Lipid metabolism
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Galactolipase, also known as PNLIPRP2, is a lipase with broad substrate specificity. It can hydrolyze both phospholipids and galactolipids. Galactolipase acts preferentially on monoglycerides, phospholipids and galactolipids. It also hydrolyses milk fat with a lower catalytic efficiency. The expressed galactolipase shows a lipolytic activity that is, however, only marginally dependent on the presence of colipase. The lipolytic activity of pancreatic extracts and human pancreatic juice on Labrasol is mainly due to the combined action of carboxyl ester hydrolase and galactolipase.
Reference	Andersson EL, et al. (2011) BSSL and PLRP2: key enzymes for lipid digestion in the newborn examined using the Caco-2 cell line. J Lipid Res. 52(11):1949-56. Xiao X, et al. (2011) Pancreatic lipase-related protein-2 (PLRP2) can contribute to dietary fat digestion in human newborns. J Biol Chem. 286(30):26353-63. Alves BN, et al. (2009) Lipid-dependent cytotoxicity by the lipase PLRP2 and by PLRP2-positive cytotoxic T lymphocytes (CTLs). Cell Biochem Funct. 27(5):296-308.