Human Gastric intrinsic factor / GIF Protein (His Tag)
IF,IFMH,INF,TCN3
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| Catalog Number | P13544-H08H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | IF,IFMH,INF,TCN3 |
| Molecular Weight | The recombinant human GIF consists of 410 amino acids and predicts a molecular mass of 44.8 KDa. It migrates as an approximately 49 KDa band in SDS-PAGE under reducing conditions. |
| predicted N | Ser 19 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human GIF (P27352-1) (Met1-Tyr417) was expressed with a polyhistidine tag at the C-terminus. |
| Bio-activity | |
| Research Area | |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Gastric intrinsic factor, also known as GIF, belongs to the of the cobalamin transport protein family. It is a glycoprotein produced by the parietal cells of the stomach. Gastric intrinsic factor plays a key role in the absorption of vitamin B12 on in the small intestine. Vitamin B12 bounds to haptocorrin after entry into the stomach. The resulting complex enters the duodenum, where pancreatic enzymes digest haptocorrin. In the less acidic environment of the small intestine, B12 can then bind to gastric intrinsic factor. This new complex travels to the ileum, where special epithelial cells endocytose them. Inside the cell, B12 dissociates once again and binds to another protein, transcobalamin II. The new complex can exit the epithelial cells to enter the liver. |
| Reference |