Human Granulin / GRN / Progranulin Protein (His Tag)
CLN11,GEP,GP88,Granulin,PCDGF,PEPI,PGRN,Progranulin
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| Catalog Number | P10826-H08H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | CLN11,GEP,GP88,Granulin,PCDGF,PEPI,PGRN,Progranulin |
| Molecular Weight | The secreted recombinant human GRN comprises 587 amino acids with a predicted molecular mass of 63.2 kDa. As a result of glycosylation, rhGRN migrates as an approximately 100-105 kDa band in SDS-PAGE under reducing conditions. |
| predicted N | Thr 18 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human GRN (NP_002078.1) (Met 1-Leu 593) with a C-terminal polyhistidine tag was expressed. |
| Bio-activity | |
| Research Area | Developmental Biology |Organogenesis |Excretory system development |Renal Markers |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | &Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth. Granulin expression inhibited Tat transactivation, and tethering experiments showed that this effect was due, at least in part, to a direct action on cyclin T1 in the absence of Tat. |
| Reference |