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Human HAI-1 / SPINT1 Protein (His Tag)

HAI,HAI1,MANSC2,SPINT1,UNQ223/PRO256

Catalog Number P11742-H08H
Organism Species Human
Host Human Cells
Synonyms HAI,HAI1,MANSC2,SPINT1,UNQ223/PRO256
Molecular Weight The recombinant human SPINT1 consists of 409 amino acids and predictes a molecular mass of 45.8 kDa. In SDS-PAGE under reducing conditions, rh SPINT1 migrates as an approximately 55 kDa band due to glycosylation.
predicted N Gly 36
SDS-PAGE
Purity > 96 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the human SPINT1 isoform 2 (O43278-2) (Met 1-Val 433) was fused with a polyhistidine tag at the C-terminus.
Bio-activity Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (R&D Systems, Catalog # ES002). IC50 value is < 2nM .
Research Area
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference