Human HNRNPR / HNRNP-R / HNRNP R Protein (His & GST Tag)

hnRNP-R,HNRNPR,HNRPR

100ug (NPP2186) Please inquiry

Catalog Number	P14309-H20B
Organism Species	Human
Host	Baculovirus-Insect Cells
Synonyms	hnRNP-R,HNRNPR,HNRPR
Molecular Weight	The recombinant human HNRNPR /GST chimera consists of 872 amino acids and has a calculated molecular mass of 98.9 kDa. The recombinant protein migrates as an approximately 114 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human HNRNPR (O43390-2) (Ala2-Lys636) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Bio-activity
Research Area	Epigenetics \|DNA / RNA \|RNA Processing \|RNA splicing
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 0.5mM GSH, pH 8.5, 3mM DTT, 10% glycerol 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	HNRNP R, also known as HNRNPR, is a RNA binding protein. HNRNP R gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). HnRNPs complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. HNRNP R has three repeats of quasi-RRM domains that bind to RNAs and also contains a nuclear localization motif.
Reference	Rossoll, et al. (2002) Specific interaction of Smn, the spinal muscular atrophy determining gene product, with hnRNP-R and gry-rbp/hnRNP-Q: a role for Smn in RNA processing in motor axons?. Hum Mol Genet. 11 (1):93-105. Mourelatos, Z, et al. (2001) SMN interacts with a novel family of hnRNP and spliceosomal proteins. EMBO J. 20(19):5443-52. Hassfeld W, et al. (1998) Molecular definition of heterogeneous nuclear ribonucleoprotein R (hnRNP R) using autoimmune antibody: immunological relationship with hnRNP P. Nucleic Acids Res. 26(2):439-45.