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Human Hemopexin / HPX Protein (His Tag)

HX

Catalog Number P10870-H08H
Organism Species Human
Host Human Cells
Synonyms HX
Molecular Weight The secreted recombinant human HPX comprises 450 amino acids with a predicted molecular mass of 50.7 kDa. As a result of glycosylation, rhHPX migrates as an approximately 70-75 kDa band in SDS-PAGE under reducing conditions.
predicted N Thr 24
SDS-PAGE
Purity > 96 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the human HPX (NP_000604.1) (Met 1-His 462) with a C-terminal polyhistidine tag was expressed.
Bio-activity Measured by its ability to bind protoporphyrin IX (PPPIX). Recombinant human Hemopexin binds > 10 μM PPPIX, resulting in a 50% decrease in the fluorescence signal of human Hemopexin .
Research Area Cancer |Signal transduction |Metabolism |Types of disease |Metabolism in Cancer
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference