Human Hexosaminidase A / HEXA Protein (Subunit A, His Tag)

TSD

Catalog Number	P12037-H08B
Organism Species	Human
Host	Baculovirus-Insect Cells
Synonyms	TSD
Molecular Weight	The recombinant human HEXA consists of 517 amino acids and predicts a molecular mass of 59.7 kDa. It migrates as an approximately 64 kDa band in SDS-PAGE under reducing conditions.
predicted N	Leu 23
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human HEXA (AAD13932.1) (Met 1-Thr 529) was fused with a polyhistidine tag at the C-terminus.
Bio-activity	Measured by its ability to hydrolyze 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide (4-MU-Glc-NAc). The specific activity is >1,250 pmoles/min/μg.
Research Area	Immunology \|Innate Immunity \|Lysosomal Enzymes
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Norflus,F. et al., 1996, DNA Cell Biol. 15 (2):89-97. Kaufman M., et al., 1997, Hum. Mutat. 10:295-300. Tanaka A., et al., 2003, J. Hum. Genet. 48:571-574. Chen R., et al., 2009, J. Proteome Res. 8:651-661. Burkard T.R., et al., 2011, BMC Syst. Biol. 5:17-17.