Human IDH1 Protein (His Tag)

HEL-216,HEL-S-26,IDCD,IDH,IDP,IDPC,PICD

• 100ug (NPP2258) Please inquiry

Catalog Number	P12055-H08E
Organism Species	Human
Host	E. coli
Synonyms	HEL-216,HEL-S-26,IDCD,IDH,IDP,IDPC,PICD
Molecular Weight	The recombinant human IDH1 consisting of 424 amino acids and has a calculated molecular mass of 48 kDa. It migrates as an approximately 43 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met 1
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human IDH1 (O75874) (Met 1-Leu 414) was fused with a polyhistidine tag at the C-terminus.
Bio-activity
Research Area	Signaling |Signal Transduction |Other Related Intracellular Topics |Cellular Senescence and Pathways in Aging |Apoptosis |Oxidative Stress |
Formulation	Lyophilized from sterile 50mM Tris, 200mM NaCl, 1mM DTT, 10% Glycerol, pH 7.5 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Takano S, et al. (2011) Detection of IDH1 mutation in human gliomas: comparison of immunohistochemistry and sequencing. Brain Tumor Pathol. 28(2): 115-23. Geisbrecht BV, et al. (1999) The human PICD gene encodes a cytoplasmic and peroxisomal NADP(+)-dependent isocitrate dehydrogenase. J Biol Chem. 274(43): 30527-30533. Nekrutenko A, et al. (1998) Cytosolic isocitrate dehydrogenase in humans, mice, and voles and phylogenetic analysis of the enzyme family. Mol Biol Evol. 15(12): 1674-1684. Henke B, et al. (1998) IDP3 encodes a peroxisomal NADP-dependent isocitrate dehydrogenase required for the beta-oxidation of unsaturated fatty acids. J Biol Chem. 273(6): 3702-3711. Gabriel JL, et al. (1986) Activity of purified NAD-specific isocitrate dehydrogenase at modulator and substrate concentrations approximating conditions in mitochondria. Metabolism. 35(7): 661-667.