Human IL-1 beta / IL1B Protein (pro form, His Tag)
IL-1,IL-1 beta,IL-1B,IL1-BETA,IL1F2
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Catalog Number | P10139-H07E |
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Organism Species | Human |
Host | E. coli |
Synonyms | IL-1,IL-1 beta,IL-1B,IL1-BETA,IL1F2 |
Molecular Weight | The recombinant pro form of human IL1β consisting of 280 amino acids and has a calculated molecular mass of 32.3 kDa. It migrates as an approximately 34 kDa band in SDS-PAGE under reducing conditions. |
predicted N | Met |
SDS-PAGE | |
Purity | > 95 % as determined by SDS-PAGE |
Protein Construction | A DNA sequence encoding the pro form of human IL1β (NP_000567.1) (Met 1-Ser 269) was fused with a polyhistidine tag at the N-terminus. |
Bio-activity | 1. Measured by its binding ability in a functional ELISA. 2. Immobilized human IL1B at 10 μg/mL (100 μl/well) can bind human IL1R1. The EC50 of human IL1R1 is 0.198 μg/mL. |
Research Area | Cardiovascular |Atherosclerosis |Vascular Inflammation |Inflammatory mediators |
Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
Background | Interleukin-1 beta (IL1 beta or IL1B) also known as catabolin, is a member of the interleukin 1 cytokine family. IL1 is a pleiotropic cytokine. It is involved in the inflammatory response, cell growth, and tissue repair in the cortex. The IL1 superfamily consists of three members, IL1A (IL1 alpha), IL1B (IL1 beta), and IL1 receptor antagonist (IL1Ra). In clinical, it has been reported that Interleukin (IL)-1 may influence Th1 / Th2 immune responsiveness and has been implicated in the establishment of successful pregnancy. Proinflammatory interleukin (IL)-1 gene polymorphisms associated with high levels of IL-1beta activity increase the risk for hypochlorhydria and distal gastric carcinoma. IL1B polymorphisms may be involved in susceptibility to SSc. Moreover, the IL2-384-G allele may be a marker for the limited phenotype of systemic sclerosis (SSc). |
Reference |