Human IL-12 (IL12A & IL12B Heterodimer) Protein
Interleukin-12
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| Catalog Number | PCT011-H08H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | Interleukin-12 |
| Molecular Weight | The recombinant IL12 heterodimer of human IL12A/IL12B comprises 525 (208 + 317) amino acids and has a calculated molecular mass of 60.2 (24 + 36.2) kDa. The apparent molecular mass of rh IL12 heterodimer is approximately 40 & 43 kDa respectively in SDS-PAGE under reducing conditions. |
| predicted N | Arg 23 & Ile 23 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the p35 subunit of human IL-12, termed as IL12A (NP_000873.2) (Met 1-Ser 219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; A DNA sequence encoding the p40 subunit of human IL-12, termed as IL12B (NP_002178.2) (Met 1-Ser 328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified. |
| Bio-activity | 1. Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA. 2. Measured in a cell proliferation assay using Anti-CD28 and Anti-CD3-stimulated PBMC. The ED50 for this effect is typically 0.1-0.6 ng/mL. |
| Research Area | Immunology |Cytokines & Growth Factors |Cytokine & Receptor |Interleukin & Receptor |IL-12 family & Receptor |IL-12 family ligand | |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | |
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