Human IL-1RA / IL1RN Protein
DIRA,ICIL-1RA,IL-1ra,IL-1ra3,IL-1RN,IL1F3,IL1RA,IRAP,MVCD4
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| Catalog Number | P10123-HNAE |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | DIRA,ICIL-1RA,IL-1ra,IL-1ra3,IL-1RN,IL1F3,IL1RA,IRAP,MVCD4 |
| Molecular Weight | The recombinant human IL1RA consists of 153 amino acids and predicts a molecular mass of 17.3 kDa which is also estimated by SDS-PAGE. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 97 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mature form of human IL1RA isoform1 (NP_776214.1) (Arg 26-Glu 177) was expressed. |
| Bio-activity | 1. Measured by its binding ability in a functional ELISA. Immobilized human IL1RA at 10 μg/ml (100 μl/well) can bind human IL1R2-Fc (P10111-H02H), The EC50 of human IL1R2-Fc (P10111-H02H) is 0.04-0.1 μg/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized human IL1RA at 10 μg/ml (100 μl/well) can bind human IL1R1-Fc (P10126-H02H), The EC50 of human IL1R1-Fc (P10126-H02H) is 0.08-0.2 μg/mL. |
| Research Area | Neuroscience |Neurology process |Neuroinflammation |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Interleukin-1 receptor antagonist (IL-1RA) also known as IL1RN is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. A polymorphism of this protein encoding gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. IL-1RA/IL1RN may inhibit the activity of IL-1 by binding to its receptor and it has no IL-1 like activity. Genetic variation in IL-1RA/IL1RN is associated with susceptibility to microvascular complications of diabetes type 4 (MVCD4). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Defects in IL-1RA/IL1RN are the cause of interleukin 1 receptor antagonist deficiency (DIRA) which is also known as deficiency of interleukin 1 receptor antagonist. Autoinflammatory diseases manifest inflammation without evidence of infection, high-titer autoantibodies, or autoreactive T-cells. DIRA is a rare, autosomal recessive, genetic autoinflammatory disease that results in sterile multifocal osteomyelitis, and pustulosis from birth. |
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