Human IL-29 / Interleukin-29 Protein (His Tag)

IFNL1,IL-29,IL29

100ug (NPP1731) Please inquiry

Catalog Number	P12339-H08H
Organism Species	Human
Host	Human Cells
Synonyms	IFNL1,IL-29,IL29
Molecular Weight	The recombinant human IL29 consists of 192 amino acids and has a predicted molecular mass of 21.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh IL29 is approximately 30-35 kDa due to glycosylation.
predicted N	Gly 20
SDS-PAGE
Purity	> 92 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human IL29 (NP_742152.1) (Met 1-Thr 200) was fused with a polyhistidine tag at the C-terminus.
Bio-activity	Measured in antiviral assays using HepG2 cells infected with vesicular stomatitisvirus (VSV). The ED₅₀ for this effect is typically 4-20 ng/ml.
Research Area	Immunology \|Cytokines & Growth Factors \|Cytokine & Receptor \|Interferon & Receptor \|Interferon
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Interleukin-29(IL-29), also known as cytokine Zcyto21, Interferon lambda-1, IFN-lambda-1, and IFNL1, is a secreted protein which belongs to the IL-28 / IL-29 family. IL-29 is a cytokine with immunomodulatory activity. IL-29 is highly similar in amino acid sequence to the IL-28. IL-28 and IL-29 are induced by viral infection and showed antiviral activity. IL-28 and IL-29 interacted with a heterodimeric class II cytokine receptor that consisted of IL-10 receptor beta (IL-10Rbeta) and an orphan class II receptor chain, designated IL-28Ralpha. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses. IL-29 may play a role in antiviral immunity. IL-29 up-regulates MHC class I antigen expression. It is a Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand / receptor complex seems to signal through the Jak-STAT pathway.
Reference	Li MC, et al. (2006) Liposome-mediated IL-28 and IL-29 expression in A549 cells and anti-viral effect of IL-28 and IL-29 on WISH cells. Acta Pharmacol Sin. 27(4): 453-9. Wang J, et al. (2009) Differentiated human alveolar type II cells secrete antiviral IL-29 (IFN-lambda 1) in response to influenza A infection. J Immunol. 182(3): 1296-304. Jordan WJ, et al. (2007) Modulation of the human cytokine response by interferon lambda-1 (IFN-lambda1/IL-29). Genes Immun. 8(1): 13-20.