Human IL-3 / Interleukin-3 Protein (His Tag)
IL-3,Interleukin-3,MCGF,MULTI-CSF
- 100ug (NPP1732) Please inquiry
| Catalog Number | P11858-H08B |
|---|---|
| Organism Species | Human |
| Host | Baculovirus-Insect Cells |
| Synonyms | IL-3,Interleukin-3,MCGF,MULTI-CSF |
| Molecular Weight | The recombinant human IL3 consists of 143 amino acids and predicts a molecular mass of 16.5 kDa. It migrates as an approximately 19 kDa band in SDS-PAGE under reducing conditions. |
| predicted N | Ala 20 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human IL3 (NP_000579.2) (Met 1-Phe 152) was expressed, fused with a polyhistidine tag at the C-terminus. |
| Bio-activity | 1. Measured in a cell proliferation assay using TF1 human erythroleukemic cells (Kitamura, T. et al. 1989 J. Cell Physiol. 140: 323.) . The ED50 for this effect is typically 0.3-1.5 ng/ml . 2. Measured by its binding ability in a functional ELISA . Immobilized human IL3 at 20 μg/ml (100 μl/well) can bind human IL3Ra with a linear ranger of 0.064-1.6 μg/ml. Immobilized human IL3 at 20 μg/ml (100 μl/well) can bind biotinylated human IL3Ra with a linear ranger of 0.32-1.6 μg/ml. |
| Research Area | Immunology |Cytokines & Growth Factors |Cytokine & Receptor |Interleukin & Receptor |Other Interleukin & Receptor |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | IL3 (interleukin 3), also known as IL-3, is a potent growth promoting cytokine which belongs to the IL-3 family. IL3/IL-3 also belongs to the group of interleukins. Interleukins are produced by a wide variety of body cells. The function of the immune system depends in a large part on interleukins, and rare deficiencies of a number of them have been described, all featuring autoimmune diseases or immune deficiency. The majority of interleukins are synthesized by helper CD4+ T lymphocytes, as well as through monocytes, macrophages, and endothelial cells. They promote the development and differentiation of T, B, and hematopoietic cells. IL3/IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL3/IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. |
| Reference |