Human IL1R1 / CD121a Protein (His Tag)
CD121A,D2S1473,IL-1 RI,IL-1R-alpha,IL-1R1,IL1R,IL1RA,P80
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| Catalog Number | P10126-H08H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | CD121A,D2S1473,IL-1 RI,IL-1R-alpha,IL-1R1,IL1R,IL1RA,P80 |
| Molecular Weight | The soluble form of recombinant human IL1R1 consists of 327 amino acids after removal of the signal peptide and has a predicted molecular mass of 37.7 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 55-60 kDa due to glycosylation. |
| predicted N | Leu 18 |
| SDS-PAGE |  |
| Purity | > 97 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain of human IL1R1 (NP_000868.1) (Met 1-Thr 332) was expressed with a fused polyhistidine tag at C-terminus. |
| Bio-activity | Measured by its ability to bind human IL1-beta in a functional ELISA. |
| Research Area | Immunology |Inflammation / Inflammatory Mediator |Inflammatory Disorders Therapeutic Targets |Rheumatoid arthritis Therapeutic Targets |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Interleukin 1 receptor, type I (IL-1R1) also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. IL-1R1/CD121a is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA). IL-1R1/CD121a is an important mediator involved in many cytokine induced immune and inflammatory responses. This protein has been characterized by pharmacological and molecular techniques in the mouse brain. The spindle-shaped astrocytes enclose the wound, separating the healthy from damaged neural tissue. The shape change and subsequent repair processes are IL-1β activity-dependent, acting through the IL-1 type 1 receptor (IL-1R1), as co-application of the IL-1type 1 receptor antagonist protein (IL-1ra) blocks IL-1β induced effects. In the spleen, a slight increase in IL-1R AcP and IL-1R1 was observed during the first hours following LPS stimulation. In conclusion, IL-1R AcP mRNA is expressed in the brain and in other tissues where IL-1R1/CD121a transcripts are found. However, the regulation of its expression is distinct from IL-1R1/CD121a. The high level of expression and the lack of regulation of IL-1R AcP transcripts in the brain under inflammatory conditions suggest that the protein might be constitutively expressed in excess. |
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