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Human IL1RL1 / ST2 Protein (isoform a, His Tag)

DER4,FIT-1,IL33R,IL‑1 R4,ST2,ST2L,ST2V,T1

Catalog Number P13034-H08H
Organism Species Human
Host Human Cells
Synonyms DER4,FIT-1,IL33R,IL‑1 R4,ST2,ST2L,ST2V,T1
Molecular Weight The recombinant human IL1RL1 consists of 321 amino acids and has a calculated molecular mass of 36.3 kDa. As a result of glycosylation, the apparent molecular mass of rh IL1RL1 is approximately 55-60 kDa in SDS-PAGE under reducing conditions.
predicted N Lys 19
SDS-PAGE
Purity > 98 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the human IL1RL1 isoform A (NP_057316.3) (Met 1-Ser 328) was expressed, fused with a polyhistidine tag at the C-terminus.
Bio-activity 1. Measured by its binding ability in a functional ELISA.
2. Immobilized recombinant human IL33 (P 10368-HNAE) at 10 μg/mL (100 μl/well) can bind biotinylated human IL1RL1 (isoform A) (P 13034-H08H) with a linear range of 0.032-8 μg/ml.
Research Area Cancer |Invasion microenvironment |Angiogenesis |Cytokines / Chemokines in Angiogenesis |Interleukins
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background IL-1 receptor–like 1 (IL1RL1) is a membrane receptor involved in TH2 inflammatory responses and eosinophilia. It has previously been described that levels of the interlukin-1 like 1 (IL1RL1) protein can be used to diagnose cardiovascular disease and determine the prognosis for a patient with cardiovascular disease. The ligand for IL1RL1 has been described, and named IL-33. Mutants in IL1RL1 have been associated with blood eosinophil counts in a genome-wide association study and with asthma in family-based and case-control studies. As an important mediator involved in many immune and inflammatory responses, this cytokine has been implicated as a regulator of both the development and effector phases of type 2 helper T cell responses, and as a negative feedback modulator of macrophage pro-inflammatory function. IL33 is a specific ligand of ST2L and induces production of Th2 cytokines.
Reference
  • Savenije OE, et al. (2011) Interleukin-1 receptor-like 1 polymorphisms are associated with serum IL1RL1-a, eosinophils, and asthma in childhood. J Allergy Clin Immunol. 127(3): 750-6.
  • Li H, et al. (2000) The cloning and nucleotide sequence of human ST2L cDNA. Genomics. 67(3): 284-90.