Human IL23R / IL23 Receptor Protein (Fc Tag)

IL23R

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Catalog Number	P13840-H02H
Organism Species	Human
Host	Human Cells
Synonyms	IL23R
Molecular Weight	The recombinant human IL23R /Fc is a disulfide-linked homodimer. The reduced monomer comprises 571 amino acids and has a predicted molecular mass of 64.9 kDa. The apparent molecular mass of the protein is approximately 92, 58 and 37 kDa in SDS-PAGE under reducing conditions.
predicted N	Gly 24
SDS-PAGE
Purity	(45+17.3+30.1) % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human IL23R (Met1-Asp353) was expressed with the Fc region of human IgG1 at the C-terminus.
Bio-activity	Measured by its binding ability in a functional ELISA. 1. Immobilized human IL23A-His+IL12B-His (PCT012-H08H) at 10 μg/ml (100 μl/well) can bind human IL23R-Fc. The EC50 of human IL23R-Fc is 0.28-0.66 μg/ml. 2. Immobilized mouse IL12Bh (m)+mIL23Ah (PCT028-M08H) at 10 μg/ml (100 μl/well) can bind human IL23R-Fc. The EC50 of human IL23R-Fc is 0.14-0.35 μg/ml.
Research Area	Immunology |Inflammation / Inflammatory Mediator |Neuroinflammation
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	IL23R, also known as IL23 receptor, belongs to the type I cytokine receptor family, Type 2 subfamily. It contains 2 fibronectin type-III domains and is expressed by monocytes, Th1, Th0, NK and dendritic cells. Isoform 1 is specifically expressed in NK cells. IL23R associates with IL12RB1 to form the interleukin-23 receptor. It binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. IL23 functions in innate and adaptive immunity and may participate in acute response to infection in peripheral tissues. IL23 may be responsible for autoimmune inflammatory diseases and be important for tumorigenesis. Genetic variations in IL23R are associated with inflammatory bowel disease type 17 (IBD17). IBD17 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. Genetic variations in IL23R also can cause susceptibility to psoriasis type 7.
Reference	Duerr RH, et al. (2006) A genome-wide association study identifies IL23R as an inflammatory bowel disease gene. Science. 314(5804):1461-3. Cargill M, et al. (2007) A large-scale genetic association study confirms IL12B and leads to the identification of IL23R as psoriasis-risk genes. Am J Hum Genet. 80(2):273-90. Dubinsky MC, et al. (2007) IL-23 receptor (IL-23R) gene protects against pediatric Crohn's disease. Inflamm Bowel Dis. 13(5):511-5. Tremelling M, et al. (2007) IL23R variation determines susceptibility but not disease phenotype in inflammatory bowel disease. Gastroenterology. 132(5):1657-64.