Human IL27Ra / TCCR / WSX1 Protein (Fc Tag)
CRL1,IL-27RA,IL27R,TCCR,WSX1,zcytor1
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| Catalog Number | P10489-H02H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | CRL1,IL-27RA,IL27R,TCCR,WSX1,zcytor1 |
| Molecular Weight | The recombinant human IL27Rα/Fc is a disulfide-linked homodimeric protein after removal of the signal peptide. The reduced monomer consists of 722 amino acids and has a predicted molecular mass of 79 kDa. As a result of glycosylation, rh IL27Rα/Fc monomer migrates as an approximately 105-115 kDa band in SDS-PAGE under reducing conditions. |
| predicted N | Gln 33 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain (Met 1-Arg 514) of human IL27Rα (NP_004834.1) was fused with the Fc region of human IgG1 at the C-terminus. |
| Bio-activity | Measured by its binding ability in a functional ELISA . Immobilized human IL27 at 20 μg/ml (100 μl/well) can bind human IL27Ra with a linear ranger of 0.064-1.6 μg/ml. |
| Research Area | Signaling |Signal Transduction |Jak/STAT Signaling |Receptors in the Jak/STAT Pathway |
| Formulation | Lyophilized from sterile PBS, pH 7.4, 8% trehalose, 8% mannitol 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | The interleukin-27 receptor is a type I cytokine receptor for interleukin-27. It is a heterodimer composed of the interleukin 27 receptor, alpha subunit and glycoprotein 130. WSX-1/IL-27R, a class I cytokine receptor that is homologous to the β2 chain of the IL-12R in both sequence and structure, is highly expressed by resting/naive CD4+ T cells and CD8+ T cells. WSX-1/IL-27R belongs to the IL-6/IL-12 family of cytokines and induced proliferation of naive CD4(+) T cells and the generation of a Th1-type adaptive immune response. WSX-1/IL-27R functions as a receptor for IL27. IL-27 not only contributes to the development of an adaptive immune response through its action on CD4(+) T cells, it also directly acts on cells of the innate immune system. WSX-1/IL-27R requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. It is involved in the regulation of Th1-type immune responses. IL-27R also appears to be involved in innate defense mechanisms. IL27RA is essential for transcriptional activation of STAT1 and for augmenting the induction of T-bet expression during initiation of Th1 cell differentiation. |
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