Human IL3RA / CD123 Protein (His & Fc Tag)
CD123,hIL-3Ra,IL3R,IL3RAY,IL3RX,IL3RY
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| Catalog Number | P10518-H03H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | CD123,hIL-3Ra,IL3R,IL3RAY,IL3RX,IL3RY |
| Molecular Weight | The recombinant human IL3RA/Fc is a disulfide-linked homodimer. The reduced monomer comprises 535 amino acids and has a predicted molecular mass of 61 kDa. As a result of glycosylation, the apparent molecular mass of rh IL3RA/Fc monomer is approximately 80-90 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Thr 19 |
| SDS-PAGE |  |
| Purity | > 75 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human IL3RA (NP_002174.1) extracellular domain (Met 1-Arg 305) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. |
| Bio-activity | Measured by its binding ability in a functional ELISA . Immobilized human IL3 at 20 μg/ml (100 μl/well) can bind human IL3RA with a linear ranger of 0.064-1.6 μg/ml. |
| Research Area | Cancer |Signal transduction |Protein Trafficking |Vesicle Transport |Adapters |Transmembrane | |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily. The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. The WSXWS motif of IL3RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box one motif of IL3RA is required for JAK interaction and / or activation. IL3RA represents a unique marker for primitive leukemic stem cells. Targeting of IL3RA may be a promising strategy for the preferential ablation of AML cells. Aberrant IL3RA expression is a good marker for monitoring of minimal residual disease. IL3RA is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. Recent studies have shown that interleukin-3 receptor alpha (CD123) is highly expressed on leukemia stem cells of patients with acute myeloid leukemia, and is correlated with tumor load and poor prognosis. CD123 was highly expressed in the bone marrow of the patients with myelodysplastic syndrome (MDS), significantly correlated with the proportion of bone marrow blasts, and thus might be the marker of MDS malignant clone. IL3RA is also a useful new marker for distinguishing B-cell disorders with circulating villous lymphocytes as its expression is characteristic of typical hairy cell leukemia (HCL) with high sensitivity and specificity. |
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