Human IL4R / CD124 Protein (His Tag)
CD124,IL-4RA,IL4R,IL4RA
- 100ug (NPP4006) Please inquiry
| Catalog Number | P10402-H08H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | CD124,IL-4RA,IL4R,IL4RA |
| Molecular Weight | The secreted recombinant human IL4R consists of 218 amino acids after cleavage of the signal peptide and predictes a molecular mass of 25.3 kDa. In SDS-PAGE under reducing conditions, it migrates with the apparent molecular mass of 43-48 kDa due to glycosylation. |
| predicted N | Met 26 |
| SDS-PAGE |  |
| Purity | > 98 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human IL4R (NP_000409.1) precursor (Met 1-His 232) was fused with a polyhistidine tag at the C-terminus. |
| Bio-activity | 1. Measured by its binding ability in a functional ELISA. Immobilized human IL4R-His (P10402-H08H) at 10 μg/mL (100 μl/well) can bind biotinylated human IL4 (P11846-HNAE). The EC50 of biotinylated human IL4 is 20.8-48.5 ng/mL. 2. Measured by its ability to inhibit IL4-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 1-4 ng/ml in the presence of 0.2 ng/ml Recombinant Human IL4. |
| Research Area | Cancer |Invasion microenvironment |Angiogenesis |Cytokine & Receptor |Interleukin & Receptor |Other Interleukin & Receptor | |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD124, also known as interleukin 4 receptor (IL4R), is a typeⅠ transmembrane protein that can regulate IgE antibody production in B cells through binding to interleukin 4 and interleukin 13 and promote differentiation of Th2 cells through binding to interleukin 4. The membrane-bound form of CD124 can be hydrolyzed to soluble form which can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. |
| Reference |