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Human IL5Ra / CD125 Protein (His Tag)

CD125,CDw125,HSIL5R3,IL5R

Catalog Number P10392-H08H
Organism Species Human
Host Human Cells
Synonyms CD125,CDw125,HSIL5R3,IL5R
Molecular Weight The recombinant human IL5Ra consists of 326 amino acids after removal of the signal peptide and has a predicted molecular mass of 37.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL5Ra is approximately 45-50 kDa due to glycosylation.
predicted N Asp 21
SDS-PAGE
Purity > 85 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the human IL5Ra isoform 2 (Q01344-2) (Met 1-Arg 335) was expressed, fused with a polyhistidine tag at the C-terminus.
Bio-activity 1. Measured by its binding ability in a functional ELISA.
2. Immobilized recombinant mouse IL5-his at 5 μg/mL (100 μL/well) can bind biotinylated human IL5Ra-his with a linear range of 32-4000 ng/mL.
3. Measured by its ability to inhibit IL5 dependent proliferation of TF1 human erythroleukemic cells. The ED50 for this effect is typically 0.7-2.8 μg/mL.
Research Area Cardiovascular |Angiogenesis |Cytokines / Chemokines in Angiogenesis |Interleukins
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background Interleukin 5 receptor, alpha (IL5RA) also known as CD125 (Cluster of Differentiation 125) is a subunit of the Interleukin-5 receptor. IL5RA (CD125) is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Six alternatively spliced transcript variants encoding three distinct isoforms have been reported. IL5RA (CD125) is a T-cell-derived cytokine which is particularly important in the development of asthma for the lerminal differentiation, activation and survival of committed cosinophil precursors.
Reference
  • Isobe M, et al. (1992) Localization of the gene encoding the alpha subunit of human interleukin-5 receptor (IL5RA) to chromosome region 3p24-3p26. Genomics. 14(3): 755-8.
  • Cheong HS, et al. (2005) Association analysis of interleukin 5 receptor alpha subunit (IL5RA) polymorphisms and asthma. J Hum Genet. 50(12): 628-34.
  • Isobe M, et al. (1992) Localization of the gene encoding the alpha subunit of human interleukin-5 receptor (IL5RA) to chromosome region 3p24-3p26. Genomics. 14(3): 755-8.