Human IL7RA / CD127 Protein (His & Fc Tag)
CD127,CDW127,IL-7R,IL-7R-alpha,IL7RA,ILRA
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| Catalog Number | P10975-H03H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | CD127,CDW127,IL-7R,IL-7R-alpha,IL7RA,ILRA |
| Molecular Weight | The recombinant human IL7Rα/Fc is a disulfide-linked homodimer. The reduced monomer consists of 464 amino acids and has a predicted molecular mass of 53 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh IL7Rα/Fc monomer is approximately 65-75 kDa due to glycosylation. |
| predicted N | Glu 21 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human IL7Rα (NP_002176.2) extracellular domain (Met 1-Gly 236) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. |
| Bio-activity | 1. Measured by its ability to bind biotinylated mouse TSLP-his (cat:51005-M08H) in functional ELISA. 2. Measured by its binding ability in a functional ELISA. Immobilized human IL7 (P11821-HNAE) at 10 μg/ml (100 μl/well) can bind human IL7Ra-Fch, The EC50 of human IL7Ra-Fch is 15.2-35.6 ng/ml. |
| Research Area | Immunology |Cluster of Differentiation (CD) |T Cell CD Antigen |CD Antigen (Helper T Cells) |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Interleukin 7 Receptor alpha (IL-7RA), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival. IL-7 receptor alpha (CD127) signaling is essential for T-cell development and regulation of naive and memory T-cell homeostasis. IL-7RA is critically required for the proper development and function of lymphoid cells. Therefore, the IL-7RA is critically required for the proper development and function of lymphoid cells. Studies from both pathogenic and controlled HIV infection indicate that the containment of immune activation and preservation of CD127 expression are critical to the stability of CD4(+) T cells in infection. A better understanding of the factors regulating CD127 expression in HIV disease, particularly on T(CM) cells, might unveil new approaches exploiting the IL-7/IL-7R receptor pathway to restore T cell homeostasis and promote immune reconstitution in HIV infection. Factors relevant to HIV infection that could potentially decrease CD127 expression on human CD8(+) T cells. CD127 down-regulation may be an important contributor to HIV-associated T-cell dysfunction. In addition to IL-7, IL-7RA also associates with TSLPR to form the functional receptor for thymic stromal lymphopoietin (TSLP) which indirectly regulates T cell development by modulating dendritic cell activation. Mutations in the human IL-7RA gene cause a type of severe combined immunodeficiency in which the major deficiencies are in T cell development, whereas B and NK cells are relatively normal in number. Variation in the IL7RA gene was recently found associated with multiple sclerosis (MS). The polymorphisms in the IL7RA gene is involved in MS pathogenesis and suggest that IL7RA variation may primarily affect chronic disease courses. Soluble CD127 (sCD127) appears to play an important role in the immunopathogenesis of several chronic infections, multiple sclerosis, and various cancers. |
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