Human ITGAX & ITGB2 Heterodimer Protein

CD11C, SLEB6, LAD, CD18, MF17, MFI7, LCAMB, LFA-1, MAC-1

Catalog Number	PCT017-H2508H
Organism Species	Human
Host	Human Cells
Synonyms	CD11C, SLEB6, LAD, CD18, MF17, MFI7, LCAMB, LFA-1, MAC-1
Molecular Weight	The recombinant heterodimer of human ITGAX/ITGB2 comprises 1879 (1130+718) amino acids and has a calculated molecular mass of 205 (125+80) kDa. As a result of glycosylation, the apparent molecular mass of rh ITGAX/ITGB2 heterodimer is approximately 95 &130 kDa in SDS-PAGE under reducing conditions.
predicted N	Phe 20 & Gln 21
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the extracellular domain (Met 1-Pro 1107) of human ITGAX (P20702) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asn 700) of human ITGB2 (P05107) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
Bio-activity
Research Area	Immunology \|Inflammation / Inflammatory Mediator \|Plasma Cascade Systems in Inflammation \|Complement System
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference