Human IZUMO4 / C19orf36 Protein (Fc Tag)
C19orf36,IMAGE:4215339
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Catalog Number | P13249-H02H |
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Organism Species | Human |
Host | Human Cells |
Synonyms | C19orf36,IMAGE:4215339 |
Molecular Weight | The recombinant human C19orf36/Fc comprises 438 amino acids and has a predicted molecular mass of 49.8 kDa. The apparent molecular mass of the protein is approximately 55-59 kDa in SDS-PAGE under reducing conditions. |
predicted N | Cys 18 |
SDS-PAGE | |
Purity | > 96 % as determined by SDS-PAGE |
Protein Construction | A DNA sequence encoding the human C19orf36 (Q1ZYL8) (Met1-His214) was expressed, fused with the Fc region of human IgG1 at the C-terminus. |
Bio-activity | |
Research Area | Developmental Biology |Reproduction |Fertilization |Gamete Fusion |
Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
Background | Izumo is a sperm membrane protein which plays a key role in the fusion in the mouse. It has an Immunoglobulin (Ig) domain and an N-terminal domain for which neither the functions nor homologous sequences are known. Up to now, there four members has an N-terminal domain with significant homology to the N-terminal domain of Izumo. We call this domain Izumo domain. The four proteins are Izumo 1, 2, 3, and 4. Izumo domain possesses the ability to form dimers, whereas the transmembrane domain or the cytoplasmic domain or both of Izumo 1 are required for the formation of multimers of higher order. Izumo 1-3 are transmembrane proteins expressed specifically in the testis, and Izumo 4 is a soluble protein expressed in the testis and in other tissues. Izumo 1, 3, and 4 formed protein complexes on sperm, Izumo 1 forming several larger complexes and Izumo 3 and 4 forming a single larger complex. Co-immunoprecipitation studies showed the presence of other sperm proteins associated with Izumo 1, suggesting Izumo 1 forms a multiprotein membrane complex. |
Reference |