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Human Insulin / INS Protein

IDDM,IDDM1,IDDM2,ILPR,INS,IRDN,MODY10

Catalog Number P11038-HNAY
Organism Species Human
Host Yeast
Synonyms IDDM,IDDM1,IDDM2,ILPR,INS,IRDN,MODY10
Molecular Weight The recombinant human Insulin consists of 53 amino acids and predicts a molecular mass of 6 KDa. It migrates as an approximately 6 KDa band in SDS-PAGE under reducing conditions.
predicted N Phe
SDS-PAGE
Purity > 95 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding human insulin B chain (2LGB_B)(Phe1-Lys29) and human insulin A chain (2KXK_A)(Gly1-Asn21) was expressed with a linker AAK.
Bio-activity Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells.
The ED50 for this effect is typically 0.2-1 µg/mL.
Research Area Immunology |Signal Transduction |Metabolism |Types of disease |Metabolism in Heart disease
Formulation Lyophilized from sterile PBS, pH 7.4.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference