Human Interleukin-21 / IL-21 Protein

CVID11,IL-21,Za11

100ug (NPP1728) Please inquiry

Catalog Number	P10584-HNAE
Organism Species	Human
Host	E. coli
Synonyms	CVID11,IL-21,Za11
Molecular Weight	The recombinant human IL-21 consisting of 134 amino acids and has a calculated molecular mass of 15.6 kDa. It migrates as an approximately 17 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 92 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mature form of human IL-21 isoform 1 (Q9HBE4-1) (Gln 23-Ser 155) was expressed and purified, with an initial Met.
Bio-activity	1. Measured by its ability to bind human IL-21R in a functional ELISA. 2. Measured by its ability to induce Interferon-gamma secretion by human natural killer lymphoma NK-92 cells. The ED₅₀ for this effect is typically 0.4-2 ng/ml.
Research Area	Immunology \|Inflammation / Inflammatory Mediator \|Inflammatory Cytokines & Chemoki and Receptors \|Common gamma Chain Receptor Family
Formulation	Lyophilized from sterile 20mM NaAc, 150mM NaCl, pH 5.5 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Coquet JM, et al. (2007) IL-21 is produced by NKT cells and modulates NKT cell activation and cytokine production. J Immunol. 178(5):2827-34. Wei L, et al. (2007) IL-21 is produced by Th17 cells and drives IL-17 production in a STAT3-dependent manner. J Biol Chem. 282(48):34605-10. Parrish-Novak J, et al. (2002) Interleukin-21 and the IL-21 receptor: novel effectors of NK and T cell responses. J Leukoc Biol. 72(5):856-63. 4 Kuchen S, et al. (2007) Essential role of IL-21 in B cell activation, expansion, and plasma cell generation during CD4+ T cell-B cell collaboration. J Immunol. 179(9):5886-96.