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Human JAG1 / Jagged 1 / CD339 Protein (His Tag)

AGS,AHD,AWS,CD339,HJ1,Jagged 1,JAGL1

Catalog Number P11648-H08H
Organism Species Human
Host Human Cells
Synonyms AGS,AHD,AWS,CD339,HJ1,Jagged 1,JAGL1
Molecular Weight The recombinant human JAG1 consists of 1024 amino acids and has a predicted molecular mass of 112 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh JAG1 is approximately 160-180 kDa due to glycosylation.
predicted N Gln 34
SDS-PAGE
Purity > 85 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the human JAG1 (NP_000205.1) extracellular domain (Met 1-Ser 1046) was expressed, fused with a polyhistidine tag at the C-terminus.
Bio-activity Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells.
The ED50 for this effect is typically 4-20 μg/mL.
Research Area Immunology |Signal Transduction |Notch Pathway
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background Protein Jagged 1, also known as JAG1, JAGL1 and CD339, is a single-pass type I  membrane protein which contains 1 DSL domain and 15 EGF-like domains. JAG1/Jagged 1 is widely expressed in adult and fetal tissues. The expression of JAG1/Jagged 1 is up-regulated in cervical squamous cell carcinoma. JAG1/Jagged 1 is also expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells. JAG1/Jagged 1 is a ligand for multiple Notch receptors. It is involved in the mediation of Notch signaling. JAG1/Jagged 1 may be involved in cell-fate decisions during hematopoiesis. 
Reference
  1. Oda T.et al., 1997, Nat. Genet. 16:235-242.
  2. Krantz I.D. et al., 1998, Am. J. Hum. Genet. 62:1361-1369.
  3. Li L. et al., 1998, Immunity. 8:43-55.
  4. Jones E.A. et al., 2000, J. Med. Genet. 37: 658-662.
  5. Roepke A.et al., 2003, Hum. Mutat. 21:100-100.
  6. Jurkiewicz D.et al., 2005, Hum. Mutat. 25:321-321.
  7. Warthen D.M.et al., 2006, Hum. Mutat. 27:436-443.