Human KIT / c-KIT / CD117 Protein (Fc Tag)
C-Kit,CD117,PBT,SCFR
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| Catalog Number | P11996-H02H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | C-Kit,CD117,PBT,SCFR |
| Molecular Weight | The recombinant human KIT/Fc comprises 729 amino acids and has a predicted molecular mass of 82 kDa. The apparent molecular mass of the protein is approximately 93-115 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Gln 26 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain of human KIT (P10721-2) (Met1-Thr516) was expressed with the Fc region of human IgG1 at the C-terminus. |
| Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized human KIT-Fc (P11996-H02H) at 10 μg/ml (100 μl/well) can bind biotinylated mouse KITL-His (1-189) (P50487-M08B), The EC50 of biotinylated mouse KITL-His (1-189) (P50487-M08B) is 2.87-6.71 ng/ml. |
| Research Area | Cancer |Signal transduction |Protein Kinase |Receptor Tyrosine Kinase (RTK) |
| Formulation | Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains.and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family and CSF-1/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumour in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumours and in distinguishing seminomas from embryonal carcinomas. Mutations in c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients. |
| Reference |