Human KLK3 / PSA / Kallikrein-3 Protein (His Tag)

APS,Hk3,KLK2A1,PSA

• 100ug (NPP1638) Please inquiry

Catalog Number	P10771-H08H
Organism Species	Human
Host	Human Cells
Synonyms	APS,Hk3,KLK2A1,PSA
Molecular Weight	The recombinant human KLK3 consists of 255 amino acids and has a predicted molecular mass of 28.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh KLK3 is approximately 35 kDa.
predicted N	Ala 18
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human KLK3 (P07288) (Met 1-Pro 261) was fused with a polyhistidine tag at the C-terminus.
Bio-activity	Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide(Suc-RPY-pNA). The specific activity is >100 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
Research Area	Cancer |Cancer immunology |Tumor-associated antigen
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	KLK3(Kallikrein 3), also known as Prostate Specific Antigen (PSA), kallikrein-related peptidase 3, Gamma-seminoprotein, is a secreted protein of the glandular kallikrein subfamily of serine proteases.KLK3 contains one peptidase S1 domain.KLK3 is a glycoprotein produced almost exclusively by the prostate gland. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
Reference	Koistinen H, et al. (2008) Development of peptides specifically modulating the activity of KLK2 and KLK3. Biol Chem. 389(6): 633-42. Yousef GM, et al. (2002) Kallikreins, steroid hormones and ovarian cancer: is there a link? Minerva Endocrinol. 27(3): 157-66. Parikh H, et al. (2011) Fine mapping the KLK3 locus on chromosome 19q13.33 associated with prostate cancer susceptibility and PSA levels. Hum Genet. 129(6): 675-85.