Human LMAN2 / VIP36 Protein (His Tag)

C5orf8,GP36B,VIP36

Catalog Number	P12455-H08E
Organism Species	Human
Host	E. coli
Synonyms	C5orf8,GP36B,VIP36
Molecular Weight	The recombinant human LMAN2 consisting of 289 amino acids and has a calculated molecular mass of 33.2 kDa. It migrates as an approximately 30 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human LMAN2 extracellular domain (Q12907) (As p45-Arg 322) was fused with a polyhistidine tag at the C-terminus and an initial Met at the N-terminus.
Bio-activity
Research Area	Immunology \|Signal Transduction \|Protein Trafficking \|ER Proteins
Formulation	Lyophilized from sterile 50mM Tris, pH 8.5 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Nawa D, et al. (2007) Stable interaction of the cargo receptor VIP36 with molecular chaperone BiP. Glycobiology. 17(9): 913-21. Shimada O, et al. (2003) Localization of VIP36 in the post-Golgi secretory pathway also of rat parotid acinar cells. J Histochem Cytochem. 51(8): 1057-63. Hara-Kuge S, et al. (1999) Vesicular-integral membrane protein, VIP36, recognizes high-mannose type glycans containing alpha1--2 mannosyl residues in MDCK cells. Glycobiology. 9(8): 833-9. Fullekrug J, et al. (1999) VIP36 localisation to the early secretory pathway. J Cell Sci. 112 ( Pt 17): 2813-21.