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Human LOXL2 / Lysyl oxidase homolog 2 Protein (His Tag)

LOR2,Lysyl oxidase-like 2,WS9-14

Catalog Number P11664-H08S
Organism Species Human
Host CHO Stable Cells
Synonyms LOR2,Lysyl oxidase-like 2,WS9-14
Molecular Weight The recombinant human LOXL2 consists of 760 amino acids and has a predicted molecular mass of 85.5 kDa.
predicted N Gln 26
SDS-PAGE
Purity (67+30) % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the human LOXL2 (Q9Y4K0) (Met1-Gln774) was expressed, fused with a polyhistidine tag at the C-terminus.
Bio-activity Measured by its ability to produce hydrogen peroxide during the oxidation of benzylamine.
The specific activity is >2pmol/min/μg
Research Area Immunology |Signal Transduction |Transcription Factors and Regulators |HIF Transcription Factors
Formulation Lyophilized from sterile 20 mM MES, 50 mM NaCl
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background Lysyl oxidase homolog 2, also known as Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14 and LOXL2, is a secreted protein which belongs to the lysyl oxidase family. LOXL2 contains four SRCR domains. The lysyl oxidase family is made up of five members: lysyl oxidase (LOX) and lysyl oxidase-like 1-4 ( LOXL1, LOXL2, LOXL3, LOXL4 ). All members share conserved C-terminal catalytic domains that provide for lysyl oxidase or lysyl oxidase-like enzyme activity; and more divergent propeptide regions. LOX family enzyme activities catalyze the final enzymatic conversion required for the formation of normal biosynthetic collagen and elastin cross-links. LOXL2 is expressed by pre-hypertrophic and hypertrophic chondrocytes in vivo, and that LOXL2 expression is regulated in vitro as a function of chondrocyte differentiation. LOXL2 promotes chondrocyte differentiation by mechanisms that are likely to include roles as both a regulator and an effector of chondrocyte differentiation. LOXL2 expression could also be explored as a molecular target in the prevention of breast cancer progression.
Reference
  • Peng,L. et al., 2009, Carcinogenesis. 30 (10):1660-9.
  • Hollosi,P. et al., 2009, Int J Cancer. 125 (2):318-27.
  • Rückert,F. et al., 2010, Int J Colorectal Dis. 25 (3):303-11.
  • Iftikhar,M. et al., 2011, J Biol Chem. 286 (2):909-18.