Human Leptin Receptor / LEPR / CD295 Protein (His Tag)
CD295,LEP-R,LEPRD,OB-R,OBR
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| Catalog Number | P10322-H08H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | CD295,LEP-R,LEPRD,OB-R,OBR |
| Molecular Weight | The recombinant human LEPR consists of 829 amino acids and has a predicted molecular mass of 95 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh LEPR is approximately 130-140 kDa due to glycosylation. |
| predicted N | Phe 22 |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain (Met 1-Asp 839) of human leptin receptor (NP_002294.2) was expressed, fused with a C-terminal polyhistidine tag. |
| Bio-activity | Measured by its binding ability in a functional ELISA . Immobilized human Leptin at 5 μg/ml (100 μl/well) can bind human Leptin receptor with a linear range of 0.032-4.0 μg/ml . |
| Research Area | Signaling |Signal Transduction |Metabolism |Types of disease |Metabolism in Cancer |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Leptin Receptor or CD295 belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Leptin Receptor/CD295 is a transmembrane catalytic receptors found on NPY/AgRP and alpha-MSH/CART neurons in hypothalamic nuclei. Leptin receptors (Ob-Rs) are coded for by one human gene that produces six different isoforms; Ob-Ra - Ob-Rf. Ob-Rs exist as constitutive dimers at physiological expression levels. Only the Ob-Rb isoform can transduce intracellular signals and does so through activation of the JAK2/STAT3, PI 3-K and MAPK signaling cascades. Activation of Ob-Rs mediates transcriptional regulation of the hypothalamic melanocortin pathway and downregulates endocannabinoid expression. Leptin acts via leptin receptors. Leptin resistance has been proposed as a pathophysiological mechanism of obesity. In obese individuals, Ob-Ra (which is involved in active transport of leptin across the blood-brain barrier) expression is downregulated and the individual may be unresponsive to leptin signals. Ob-R antagonists are of great interest in the development of pharmacological treatments for obesity. Mutations in Leptin Receptor/CD295 have been associated with obesity and pituitary dysfunction. |
| Reference |