Human MAPKAPK5 Protein (His & GST Tag)

MAPKAP-K5,MK-5,MK5,PRAK

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Catalog Number	P13655-H20B
Organism Species	Human
Host	Baculovirus-Insect Cells
Synonyms	MAPKAP-K5,MK-5,MK5,PRAK
Molecular Weight	The recombinant human MAPKAPK5/GST chimera consists of 708 amino acids and has a calculated molecular mass of 82 KDa. It migrates as an approximately 75KDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 76 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human MAPKAPK5 isoform 1 (NP_003659.2) (Met 1-Gln 471) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Bio-activity	Kinase activity untested
Research Area	Cancer |Oncoprotein & suppressor & biomarker |Tumor suppressor |Other in Tumor suppressor
Formulation	Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	MAPKAPK5 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family. MAPKAPK5 has significant sequence homology to mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK). It is widely distributed. MAPKAPK5 can be phosphorylated by extracellular-regulated kinase (ERK), and p38 kinase but not by c-jun N-terminal kinase (JNK)in vitro.Recombinant GST-MAPKAPK5 protein can phosphorylate a peptide derived from the regulatory light chain of myosin II. Phosphorylation of MAPKAPK5 by ERK and p38 kinase increased its activity by 9 and 15 fold respectively. Taken together, these data suggest that MAPKAPK5 is a novelin vitrosubstrate for ERK and p38 kinase. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. MAPKAPK5 also mediates stress-induced small heat shock protein 27 phosphorylation.
Reference	Ni H, et al. (1998) MAPKAPK5, a novel mitogen-activated protein kinase (MAPK)-activated protein kinase, is a substrate of the extracellular-regulated kinase (ERK) and p38 kinase. Biochem Biophys. Res Commun. 243 (2): 492-6. Sudo T, et al. (2000) p62 functions as a p38 MAP kinase regulator. Biochem Biophys Res Commun. 269 (2): 521-5. Strausberg RL, et al. (2003) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99 (26): 16899-903. New L, et al. (2004) Regulation of PRAK subcellular location by p38 MAP kinases. Mol Biol Cell. 14 (6): 2603-16.