Human MID1IP1 Protein (His Tag)

G12-like,MIG12,S14R,STRAIT11499,THRSPL

Catalog Number	P14005-H07E
Organism Species	Human
Host	E. coli
Synonyms	G12-like,MIG12,S14R,STRAIT11499,THRSPL
Molecular Weight	The recombinant human MID1IP1 consists of 198 amino acids and predicts a molecular mass of 22 KDa. It migrates as an approximately 28 KDa band in SDS-PAGE under reducing conditions.
predicted N	His
SDS-PAGE
Purity	> 85 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human MID1IP1 (Q9NPA3 ) (Met1-His183) was expressed with a polyhistidine tag at the N-terminus.
Bio-activity
Research Area	Cancer \|Signal transduction \|Metabolism \|Types of disease \|Metabolism in Cancer
Formulation	Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% Glycerol, pH 8.0. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	MID1IP1 belongs to the SPOT14 family. It is a homodimer in the absence of THRSP. MID1IP1 interacts with ACACA and ACACB. It plays a role in the regulation of lipogenesis in liver. It up-regulates ACACA enzyme activity. MID1IP1 is required for efficient lipid biosynthesis, including triacylglycerol, diacylglycerol and phospholipid. MID1IP1 is involved in stabilization of microtubules. Its interaction with THRSP interferes with ACACA binding.
Reference	Aipoalani DL. et al., 2010, Endocrinology. 151 (5): 2071-7. Colbert CL. et al., 2010, Proc Natl Acad Sci. 107 (44): 18820-5. Inoue J. et al., 2011, Mol Endocrinol. 25 (6): 995-1005.