Human MKI67 Protein (GST Tag)

KIA,MIB-,MIB-1,PPP1R105

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Catalog Number	P13180-H09E
Organism Species	Human
Host	E. coli
Synonyms	KIA,MIB-,MIB-1,PPP1R105
Molecular Weight	The recombinant human MKI67 /GST chimera consists of 354 amino acids and has a predicted molecular mass of 41 kDa. It migrates as an approximately 37-41 KDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 80 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mature form of human MKI67 (P46013-2) (Met1-Pro120) was fused with the GST tag at the N-terminus.
Bio-activity
Research Area	Epigenetics |Cell cycle |Markers
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	MKI67 contains 1 FHA domain and plays a key role in cell proliferation. During interphase, the MKI67 antigen can be exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface of the chromosomes. MKI67 protein is present during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent from resting cells. MKI67 is an excellent marker to determine the growth fraction of a given cell population. The fraction of MKI67-positive tumor cells is often correlated with the clinical course of cancer. It is also associated with ribosomal RNA transcription. Inactivation of antigen MKI67 leads to inhibition of ribosomal RNA synthesis.
Reference	Rahmanzadeh R, et al. (2007) Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis. Cell Prolif. 40(3):422-30. Bullwinkel J, et al. (2006) Ki-67 protein is associated with ribosomal RNA transcription in quiescent and proliferating cells. J Cell Physiol. 206(3):624-35. Schonk DM, et al. (1989) Assignment of the gene(s) involved in the expression of the proliferation-related Ki-67 antigen to human chromosome 10. Hum Genet. 83(3):297-9. Endl E, et al., 2000, Exp Cell Res. Jun 15;257(2): 231-7. Luporsi E, et al., 2012, Breast Cancer Res Treat. 132(3): 895-915. Scholzen T, et al., 2000, J Cell Physiol. 182(3): 311-22.