Human MKK6 / MEK6 / MAP2K6 Protein (207 Ser/Asp, 211 Thr/Asp, His & GST Tag)
MAPKK6,MEK6,MKK6,PRKMK6,SAPKK-3,SAPKK3
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| Catalog Number | P10422-H20B1 |
|---|---|
| Organism Species | Human |
| Host | Baculovirus-Insect Cells |
| Synonyms | MAPKK6,MEK6,MKK6,PRKMK6,SAPKK-3,SAPKK3 |
| Molecular Weight | The recombinant human MAP2K6(S207D&T211D）/GST chimera consists of 571 amino acids and has a calculated molecular mass of 65.3 KDa. It migrates as an approximately 60 KDa band in SDS-PAGE under reducing conditions. |
| predicted N | Met |
| SDS-PAGE |  |
| Purity | > 88 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human MAP2K6 isoform 1 (P52564-1) (Met 1-Asp 334, Ser 207/Asp, Thr 211/Asp) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
| Bio-activity | The specific activity was determined to be 1250 nmol/min/mg using inactive MAPK14 as substrate. |
| Research Area | Signaling |Signal Transduction |Protein Kinase |Intracellular Kinase |MAP2K (Mitogen-Activated Protein Kinase Kinase) |
| Formulation | Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Dual specificity mitogen-activated protein kinase kinase 6, also known as MAP kinase kinase 6, MAPKK 6, MAPK / ERK kinase 6, SAPKK3, MAP2K6 and MKK6, is a protein which belongs to the?protein kinase superfamily, STE Ser / Thr protein kinase family and MAP kinase kinase subfamily. MAP2K6 / MKK6 contains one?protein kinase domain. Mitogen-activated protein kinases are members of a conserved cascade of kinases involved in many signal transduction pathways. They stimulate phosphorylation of transcription factors in response to extracellular signals such as growth factors, cytokines, ultraviolet light, and stress-inducing agents. MAP2K6 / MKK6 exists in a variety of alternatively spliced isoforms with distinct patterns of tissue expression. Isoform 2 of MAP2K6 / MKK6 is only expressed in skeletal muscle. Isoform 1 of MAP2K6 / MKK6 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas. |
| Reference |