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Human MSLN / Mesothelin Protein (Fc Tag)

Mesothelin,MPF,SMRP

Catalog Number P13128-H02H
Organism Species Human
Host Human Cells
Synonyms Mesothelin,MPF,SMRP
Molecular Weight The recombinant human MSLN/Fc comprises 491 amino acids and has a predicted molecular mass of 53.7 kDa. The apparent molecular mass of the protein is approximately 56 kDa in SDS-PAGE under reducing conditions.
predicted N Leu 37
SDS-PAGE
Purity > 99 % as determined by SDS-PAGE
Protein Construction A DNA sequence encoding the human MSLN (Q13421-2) (Met1-Arg286) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Bio-activity
Research Area Cancer |Cancer immunology |Tumor-associated antigen
Formulation Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background Megakaryocyte potentiating factor belongs to the mesothelin family. This family is comprised by several mammalian pre-pro-megakaryocyte potentiating factor precursor (MPF) or mesothelin proteins. Mesothelin is a glycosylphosphatidylinositol-linked glycoprotein highly expressed in mesothelial cells, mesotheliomas, and ovarian cancer, but the biological function of the protein is not known. Megakaryocyte potentiating factor is highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). It interacts with MUC16 and potentiates megakaryocyte colony formation in vitro. Megakaryocyte potentiating factor is secreted by several mesothelioma cell lines and is frequently elevated in the blood of patients with mesothelioma. Measurement of this protein may be useful in following the response of mesothelioma to treatment.
Reference
  • Chang MC, et al. (2012) Mesothelin enhances invasion of ovarian cancer by inducing MMP-7 through MAPK/ERK and JNK pathways. Biochem J. 442 (2): 293-302.
  • Nelson HH, et al. (2011) The relationship between tumor MSLN methylation and serum mesothelin (SMRP) in mesothelioma. Epigenetics. 6 (8): 1029-34.
  • Bournet B, et al. (2012) Gene expression signature of advanced pancreatic ductal adenocarcinoma using low density array on endoscopic ultrasound-guided fine needle aspiration samples. Pancreatology. 12 (1): 27-34.