Human N6AMT1 / HEMK2 Protein (His Tag)

C21orf127,HEMK2,m.HsaHemK2P,MTQ2,N6AMT,PRED28

Catalog Number	P12399-H08E
Organism Species	Human
Host	E. coli
Synonyms	C21orf127,HEMK2,m.HsaHemK2P,MTQ2,N6AMT,PRED28
Molecular Weight	The recombinant human N6AMT1 comprises 196 amino acids and has a calculated molecular mass of 21.2 kDa. It migrates as an approximately 23 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met 1
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mature form of human N6AMT1 (AAH11554.1) (Met 1-Ser 186) was fused with a polyhistidine tag at the C-terminus.
Bio-activity
Research Area	Signaling \|Signal Transduction \|Metabolism \|Pathways and Processes \|Vitamins / minerals
Formulation	Lyophilized from sterile 20mM Tris, pH 8.0 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Ren X, et al. (2011) Involvement of N-6 adenine-specific DNA methyltransferase 1 (N6AMT1) in arsenic biomethylation and its role in arsenic-induced toxicity. Environ Health Perspect. 119(6): 771-7. Yang Z, et al. (2004) Structural characterization and comparative phylogenetic analysis of Escherichia coli HemK, a protein (N5)-glutamine methyltransferase. J Mol Biol. 340(4): 695-706. Bujnicki JM, et al. (1999) Is the HemK family of putative S-adenosylmethionine-dependent methyltransferases a missing zeta subfamily of adenine methyltransferases? A hypothesis. IUBMB Life. 48(3): 247-9.